A floxed neo cassette in inverse orientation was inserted into intron 1 and a loxP site was inserted into intron 3 via homologous recombination. The neo cassette was later removed leaving exons 2 and 3 floxed. (J:146078)
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A floxed neo cassette in inverse orientation was inserted into intron 1 and a loxP site was inserted into intron 3 via homologous recombination. The neo cassette was later removed leaving exons 2 and 3 floxed. (J:146078)