Exon 5 was replaced with one which contains an HA-tag. A floxed and frt-flanked neo cassette with a splice acceptor, similar to the in BayGenomics gene trap vectors, was inserted upstream of the modified exon 5. (J:146179)
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Exon 5 was replaced with one which contains an HA-tag. A floxed and frt-flanked neo cassette with a splice acceptor, similar to the in BayGenomics gene trap vectors, was inserted upstream of the modified exon 5. (J:146179)