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A targeting vector containing a self-excising loxP-flanked neomycin/sperm-specific Cre cassette was used to target a loxP site and a I-SceI endonuclease site upstream of the S-mu switching region and downstream of the E-mu enhancer. The construct was electroporated into albino C57BL/6 embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male founder had self-excision of the neomycin/Cre cassette, leaving the loxP and I-SceI sites in the germline. Correct targeting was confirmed by Southern blotting. B cell development and numbers were normal in heterozygote mice. (J:145691)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
