Hprt1^{tm17(Ple48-lacZ)Ems}

The Ple48-lacZ transgene (pEMS1519) was designed with the 1533 bp Ple48 minipromoter (DBH-A;derived from a subsection of the promoter from the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human dopamine beta-hydroxylase (dopamine beta-monooxygenase) [DBH] gene direct expression of beta-galactosidase (lacZ) in nroadrenergic nuclei and exogenous regions including the cortex. (J:144244, J:164356)