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A neomycin resistance cassette replaced part of exon 2 and all of exons 3-5. If splicing were to occur, the resulting mRNA would encode only the first 23 codons and an out of frame codon followed by a stop codon. Loss of protein expression was confirmed by immunoblot analysis. Many mice in this colony are heterozygous for a 365 kb deletion between positions 60.976 and 61.341 Mb of chromosome 6. Since this mutation is on chromosome 12, it should be possible to breed it away from the chromosome 6 deletion. (J:145048)