Id1^tm1Xhsu

An eGFP vector and a floxed neomycin cassette was knocked into the untranslated region of exon 1 of the gene locus. Founder mice were crossed with EIIa-cre transgenic mice to remove the neomycin cassette, leaving behind a loxP site. GFP expression was detected in bone marrow cells of myeloid lineages in both heterozygote and homozygote mice. Endogenous protein was not detected in immunobloting of cytokine-stimulated bone marrow cells from homozygote mice confirming this is a null allele. (J:144696)