A loxP site was inserted in the EcoRV site upstream from exon 4 and a loxP-flanked neomycin cassette was inserted downstream of exon 4. Correct targeting was confirmed by Southern blotting. (J:144416)
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A loxP site was inserted in the EcoRV site upstream from exon 4 and a loxP-flanked neomycin cassette was inserted downstream of exon 4. Correct targeting was confirmed by Southern blotting. (J:144416)