A floxed puromycin resistance gene followed by an IRES sequence and a cDNA encoding EGFP was inserted downstream of the stop codon in exon 10. Germ line, cre-mediated recombination was used to remove the puro gene. (J:144100)
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A floxed puromycin resistance gene followed by an IRES sequence and a cDNA encoding EGFP was inserted downstream of the stop codon in exon 10. Germ line, cre-mediated recombination was used to remove the puro gene. (J:144100)