Il17f^tm2Yiw

This gene locus lies within 60 kb of the highly homologous locus Il17A. In order to generate a double mutant, ES cells heterozygous for the Il17atm1Yiw null allele were retargeted with a second vector to the Il17f locus. A hygromycin resistance gene replaced exons 2 and 3 of the allele that was in cis with the Il17atm1Yiw allele. Gene inactivation of both loci was confirmed by RT-PCR of activated splenocytes collected from homozygote mice. (J:143719)