Exons 3 and 4 were flanked by loxP sites and an frt flanked neo cassette was inserted after the second loxP site via homologous recombination. The neo cassette was subsequently removed via Flp mediated recombination. (J:143922)
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Exons 3 and 4 were flanked by loxP sites and an frt flanked neo cassette was inserted after the second loxP site via homologous recombination. The neo cassette was subsequently removed via Flp mediated recombination. (J:143922)