Tg(KRT14-Artn)1Kma

The transgene consists of a purified fragment containing 2.3 kb of the human K14 keratin promoter sequence, 1.1 kb of mouse artemin DNA, and 1.7 kb of the human growth hormone gene containing intron/exon and poly(A) signal sequences. RT-PCR analysis of transgene expression showed a low level of artemin mRNA in skin from WT mice and an enhanced level in skin of ART-OE mice. At the protein level, comparison of artemin immunolabeling of WT and ART-OE skin showed only slight reactivity in the WT epidermis and a significant enhancement in artemin immunoreactivity in transgenic skin that was restricted to the K14 keratin-expressing keratinocytes. (J:111687)