A loxP site was inserted into the untranslated region of exon 1 and a floxed neo cassette was inserted downstream of exon 3. Transient cre expression in ES cells was used to remove the neo cassette leaving most of exon 1 through exon 3 floxed. (J:143473)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count