A loxP site was inserted in intron 1 and a floxed PGK-neo cassette was inserted in intron 4. The neomycin selection cassette was subsequently excised from correctly targeted ES cells by transient cre expression. (J:142321)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count