H2-T23^tm3Cant

Exon 4 of the gene locus was targeted with a mutation that causes an aspartic acid to lysine exchange at amino acid position 227 (D227K). A floxed neomycin resistance cassette was also inserted downstream of exon 5 and was subsequently removed by crossing chimeric mice with a cre transgenic strain. The mutant protein was expressed on the surface of activated CD4+ T cells from homozygous mice at levels similar to the endogenous protein in wild-type mice. This mutation was predicted to disrupt binding to the CD8 coreceptor complex. (J:142087)