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The entire coding region of the locus was replaced by a Gfp reporter gene and a loxP-flanked neomycin resistance cassette. Chimeric mice were crossed with a cre-deleter strain to remove the neo cassette, leaving behind a single loxP site. The mutated allele has the Gfp gene inserted in-frame with the endogenous start and stop codons. Fetal livers from E13.5 homozygous embryos have expression of GFP but no expression of the endogenous gene locus, as determined by immunoblot analysis. GFP expression is visible by fluorescent microscopy in both heterozygote and homozygote E13.5 embryos. (J:141749)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
