A neo site was inserted downstream of exon 2 and a floxed neo cassette downstream of exon 3. Transient cre expression in ES cells was used to remove the neo cassette leaving exon 3 floxed. (J:141633)
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A neo site was inserted downstream of exon 2 and a floxed neo cassette downstream of exon 3. Transient cre expression in ES cells was used to remove the neo cassette leaving exon 3 floxed. (J:141633)