A loxP site was inserted upstream of exon 2 and an frt-flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exons 2 through 4 floxed. (J:141391)
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A loxP site was inserted upstream of exon 2 and an frt-flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exons 2 through 4 floxed. (J:141391)