The gene trap vector pU17, which contains a beta-geo cassette flanked by loxP and lox71 sites, was inserted 215 bp upstream of exon 1. A 50% reduction in transcript expression was confirmed by real-time RT-PCR on extracts heterozygous for this allele. (J:141491)
Basic Information
(C57BL/6NCrlj x CBA/JNCrlj)F1
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count