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ES cells containing the GFP knock-in allele, Zbtb7btm1Tani, were targeted for a second mutation. A loxP site was placed approximately 6 kb upstream of the transcription start site, placing it 5' to a proximal enhancer for this gene locus. A floxed neo-cassette was placed downstream of the enhancer. Transient expression of cre-recombinase removed a 2.2-kb region including the proximal enhancer and the neo cassette. Southern blotting confirmed correct targeting of the knock in allele. (J:141142)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
