Exons 3 to 6 were replaced with a neomycin selection cassette. In addition, a stop codon was inserted in exon 2. RT-PCR of RNA extracted from the brains of homozygous mice demonstrated that no transcription occurred. (J:141037)
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Exons 3 to 6 were replaced with a neomycin selection cassette. In addition, a stop codon was inserted in exon 2. RT-PCR of RNA extracted from the brains of homozygous mice demonstrated that no transcription occurred. (J:141037)