Exon 2, which contains the ATG start codon, was floxed by the following construct. A loxP site was inserted upstream of exon 2, and a loxP site and a Frt-flanked neomycin cassette was inserted downstream of exon 2. Correct targeting was confirmed by genomic PCR. (J:140870)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count