A loxP site was inserted upstream of exon 1 and an frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 1. Transient flp expression in ES cells was used to remove the neo cassette leaving exon 1 floxed. (J:140129)
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A loxP site was inserted upstream of exon 1 and an frt-flanked neo cassette with a 5' loxP site was inserted downstream of exon 1. Transient flp expression in ES cells was used to remove the neo cassette leaving exon 1 floxed. (J:140129)