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A loxP site and a frt-flanked neomycin resistance cassette were inserted between exons 3 and 4, and a second loxP site was inserted between exons 11 and 12. Founder mice were crossed to a transgenic strain expressing Flp recombinase (Tg(ACTFLPe)9205Dym mice) to remove the neomycin cassette and leave exons 4 through 11 flanked by loxP sites. Southern blot and PCR analyses on genomic DNA confirmed that the correct targeting and recombinase events occurred. (J:140018)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
