A lxoP site was inserted upstream of the first exon. An FRT-flanked neo cassette with a 5' loxP was inserted downstream of the last exon. Flp-mediated recombination removed the neo cassette and left the 4 exons floxed. (J:138817)
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A lxoP site was inserted upstream of the first exon. An FRT-flanked neo cassette with a 5' loxP was inserted downstream of the last exon. Flp-mediated recombination removed the neo cassette and left the 4 exons floxed. (J:138817)