Tg(CAG-DsRed,-EGFP)5Gae

The CCAG-R/G expression vector was designed with (from 5' to 3') a Human cytomegalovirus immediate early enhancer and chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, a DsRed-Express protein sequence followed by three SV40 polyadenylation signals (DsRed-Express (Clontech) is a red fluorescent protein tetramer with improved solubility and fluorescence detection), a second loxP site, an enhanced green fluorescent protein (EGFP) sequence itself followed by a rabbit beta-globin polyadenylation signal. To minimize integration site effects and possible promoter interference, the CCAG-R/G expression vector was flanked on both sides with two paired copies of anti-sense oriented 5'-HS4 insulator sequences from the chicken beta-globin gene; generating the final "insulator/red/green" targeting vector (pIRG). Line 5 was established and has an estimated transgene copy number of 7. Embryonic and adult tissues exhibit widespread red fluorescent protein expression in the absence of cre recombinase. (J:137251)