A loxP flanked neo cassette and a loxP site were introduced by homologous recombination into introns 1 and 3 respectively. The neo cassette was excised by transient expression of cre recombinase in vitro leaving exons 2 and 3 floxed. (J:138991)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count