A loxP flanked neo cassette and a loxP site were introduced by homologous recombination into introns 1 and 3 respectively. The neo cassette was excised by transient expression of cre recombinase in vitro leaving exons 2 and 3 floxed. (J:138991)
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A loxP flanked neo cassette and a loxP site were introduced by homologous recombination into introns 1 and 3 respectively. The neo cassette was excised by transient expression of cre recombinase in vitro leaving exons 2 and 3 floxed. (J:138991)