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The coding sequence found within the intron of another gene was replaced with an frt- and loxP-flanked neo cassette. The selection cassette was subsequently removed by germ line, cre mediated recombination. Normal expression of the surrounding gene was confirmed by western blot analysis on heart extracts. The absence of the targeted sequence transcript was confirmed by northern blot analysis on lung and heart extracts. (J:139708)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
