Gt(ROSA)26Sor^{tm6(CAG-ZsGreen1)Hze}

The Rosa-CAG-LSL-ZsGreen1-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), ZsGreen1 sequence (from pZsGreen1-N1 (Clontech); a human codon optimized/enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:155793)