The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 6904bp between positions 27380692-27387595 of Chromosome 13 (Build GRCm39). The entire coding sequence was replaced with an in-frame beta-galactosidase (lacZ) cassette (inserted at the ATG codon in the first exon) followed by a neomycin resistance cassette. (J:136110, J:256588)