A loxP site was inserted after the first coding exon and a floxed neo cassette was inserted after the last coding exon. Expression of cre in ES cells was used to remove the neo cssette leaving coding exons 2 through 4 floxed. (J:139170)
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A loxP site was inserted after the first coding exon and a floxed neo cassette was inserted after the last coding exon. Expression of cre in ES cells was used to remove the neo cssette leaving coding exons 2 through 4 floxed. (J:139170)