Exon 2 was replaced with an in frame EGFP cassette, exon 2 and floxed neo cassette. Transient cre expression in ES cells was used to remove the neo cassette. Expression of the EGFP precludes expression of the endogenous gene. (J:138039)
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Exon 2 was replaced with an in frame EGFP cassette, exon 2 and floxed neo cassette. Transient cre expression in ES cells was used to remove the neo cassette. Expression of the EGFP precludes expression of the endogenous gene. (J:138039)