Exons 3 and 4 were replaced by a floxed neomycin selection cassette. Chimeric mice were crossed to a CMV-cre transgenic mouse to excise the neo cassette in the germline. Mice were backcrossed to C57BL/6 more than five times. (J:138057)
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Exons 3 and 4 were replaced by a floxed neomycin selection cassette. Chimeric mice were crossed to a CMV-cre transgenic mouse to excise the neo cassette in the germline. Mice were backcrossed to C57BL/6 more than five times. (J:138057)