Napepld^tm1.1Rpa

LoxP sites flank exon 3. An Sv-Neo (SV40 promoter-neomycinphosphotransferase) gene is flanked by frt sites from removal by the action of FLPase. After removal of the Sv-Neo cassette by breeding with FLPer mice, exon 3 was removed in all cells by breeding with Mox2-Cre mice to generate heterozygous animals with one null NAPE-PLD allele. These mice were bred with C57BL/6 mice to remove the Mox2-Cre gene, and then bred together to generate mice homozygous for the NAPE-PLD-null allele. (J:132692)