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A 800-kb yeast artificial chromosome (YAC) was constructed from human germline sequence of the immunoglobulin kappa light chain gene cluster. This YAC consisted of 32 V-kappa gene segments, all 5 J segments, the C-kappa constant region, the downstream kappa deleting element and a neomycin resistance cassette. Tw transgenic lines were created with one, J23.1, being propagated for further analysis. Southern blot analysis confirmed that the YAC inserted without any recombination events. Transgenic mice were found to express human kappa light chain immunoglobulin. (J:70620, J:139719)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
