A loxP site was inserted upstream of exon P2 and a neo cassette with a 3' loxP site was inserted downstream of exon P2. Transient cre expression in fertilized oocytes was used to remove the neo cassette and exon P2. (J:137666)
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A loxP site was inserted upstream of exon P2 and a neo cassette with a 3' loxP site was inserted downstream of exon P2. Transient cre expression in fertilized oocytes was used to remove the neo cassette and exon P2. (J:137666)