Tg(Fabp1-GH1)5Bir

This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -4000 to +21 from the rat liver fatty-acid binding protein gene; the insertion also contains the pUC13 vector. Mice of this line (from founder G0#1) have ~66 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal intestinal expression gradient, highest in the proximal small intestine and declining progressively, though less rapidly for hGH than for Fabp1, through the distal colon. This Fabp1 5' regulatory segment suppresses the expression observed in colon and kidney of mice bearing similar transgenes with a shorter promoter (See Tg(Fabp1-GH1)7Bir, Tg(Fabp1-GH1)10Bir). (J:109939)