A lox P site was inserted upstream of exon 1 and an frt-flanked neo cassette with a 3' loxP site was inserted downstream of exon 1. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exon 1 floxed. (J:137502)
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A lox P site was inserted upstream of exon 1 and an frt-flanked neo cassette with a 3' loxP site was inserted downstream of exon 1. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exon 1 floxed. (J:137502)