Rnf8^{Gt(RRR260)Byg}

The gene trap vector is inserted after exon 4, disrupting proper transcription of the gene and generating a fusion product of beta-geo and the N-terminal part of the encoded protein without its C-terminal RING domain which is critical for function. The absence of protein expression was confirmed by western blot analysis on thymocyte extracts. (J:137023, J:159114, J:161240)