he Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The neomycin fragment may have been removed. (J:137340)