A loxP site was inserted into intron 4 and an FRT and loxP site flanked neomycin resistance gene cassette was inserted onto intron 6. Flp-mediated recombination was used to remove the neo cassette leaving exons 5 and 6 floxed. (J:137138)
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A loxP site was inserted into intron 4 and an FRT and loxP site flanked neomycin resistance gene cassette was inserted onto intron 6. Flp-mediated recombination was used to remove the neo cassette leaving exons 5 and 6 floxed. (J:137138)