Tg(PSCA-EGFP)1Witt

A transgenic vector carrying 9 kb of the 5' flanking sequence and 5 bp of the untranslated region of human prostate stell cell antigen driving expression of enhanced green fluorescent protein (EGFP) was introduced into (C57BL/6 x C3H)F1 embryos. Expression is detected in mid-gestation following the appearance of prostatic buds from the urogenital sinus. In adult mice, EGFP expression is restricted to a subset of cells located in the distal tips of the prostate gland towards the urethra. GFP expression increases during puberty and regeneration driven by androgen which are associated with expansive growth of the prostate. Transient GFP expression was detected in the most superficial epidermal skin layer embryonically, but was extinguished with age. No other organs or tissues exhibited detectable transgenic protein expression. (J:73560)