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A rat Erbb2 cDNA containing an activating mutation and point mutations in the catalytic domain is expressed under the control of the MMTV LTR with SV40 polyadenylation and splicing signals. The activating mutation, derived from a clone isolated from mouse mammary tumor tissue, consists of a 12 codon in-frame deletion (neu8142 or NDL1) in the region encoding the extracellular region directly adjacent to the transmembrane domain. In addition, sequences encoding the tyrosine kinase domain were altered to mutate residues 1028 (site A), 1144 (site B), 1201 (site C), 1226/1227 (site D), and 1253 (site E) from tyrosine to phenylalanine. Expression of the transgene in mammary tissue was confirmed by RT-PCR. (J:67363)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
