An frt-flanked betageo cassette with a 3' loxP site and a 5' SV40-polyA tail was inserted downstream of exon 8 and an additional loxP site was inserted upstream of exon 8. Transient flp expression in ES cells was used to remove the betageo cassette and leave exon 8 floxed. (J:134633)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count