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A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring. (J:134516)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
