This allele was created to facilitate insertion of vectors into the Rosa26 locus using recombinase-mediated cassette exchange. A splice acceptor followed by a pgk promoter driving an attP-flanked hygromycin selection gene was inserted into intron 1. Upon expression of C31 intergrase, the hyrgromycin cassette will be exchanged with a donor vector containing an attB-flanked cassette. (J:133638)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6J x 129S6/SvEvTac)F1
Targeted
Insertion
--
--
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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