This allele was created to facilitate insertion of vectors into the Rosa26 locus using recombinase-mediated cassette exchange. A splice acceptor followed by a pgk promoter driving an attP-flanked hygromycin selection gene was inserted into intron 1. Upon expression of C31 intergrase, the hyrgromycin cassette will be exchanged with a donor vector containing an attB-flanked cassette. (J:133638)
Basic Information
(C57BL/6J x 129S6/SvEvTac)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count