Pkd1l3^tm1Sul

A 4.1 kb PacI-PacI genomic fragment of the gene including exons 17-21 was replaced with a 2.0 kb neomycin resistance cassette. The cassette was flanked by Frt and loxP sites and expression is driven by the pGKEM7 promoter: Frt-loxP-pGKEM7neo-Frt-loxP. This eliminates transmembrane domains 2 through 5 and causes a downstream shift in reading frame with consequent early stop codons. The predicted truncated protein lacks the ion channel pore and is therefore non functional. No expression was detected when the mice were examined by in situ hybridization of the taste buds. (J:134024, J:168530)