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A targeting vector was used to place a loxP site in the 5' UTR of the gene in the AflII restriction site 70 bp 5' of the ATG. A tetracysteine tag was inserted in frame into the first coding exon (from GGLSGSS to GGLSGCCPGCCSGSS) and an frt-neo-frt-loxp cassette was inserted 110 bp downstream of exon 1 into the genomic NdeI site. The line was crossed to cre-transgenic mice to excise the first coding exon. Total brain protein levels are normal. Rims1 is 30% more soluble in a detergent-free fractionation. (J:133656)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
