App^tm1Sud

The targeting vector incorporates a wildtype mouse App exon 16 (truncated after residue KM) followed by a FLAG tag (2 repeats), a stop codon, a poly A signal region from the human growth hormone gene, and a pair of neomycin resistance cassettes which are flanked by a pair of FRT sites. All of these elements are flanked by a single pair or loxP sites. This construct is followed by an additional copy of exon 16 carrying the Swedish mutation and modified version of exon 17 carrying the London mutation plus the Dutch mutation. Mice were crossed with an FLP strain (unspecified background) to remove the neomycin cassettes. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. (J:133653)