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The entire 1.4 Kb cDNA from a human AKR1B1 (hAR) clone with 36 bo 5' and 325 bp 3' untranslated regions was cloned into a vector containing the SV40 splice site and poly(A) sequence. The -341 to +49 region of the mouse Cryaa promoter was inserted at the 5' end of the AKR1B1 sequence and microninjected into fertilized oocytes. Northern blot analysis showed expression of AKR1B1 in the lens, but not in liver, brain, testis, or muscle. Five lines were characterized, CAR222, -223. -435, -440, and -648 with 30, 70, 100, 70, and 5 copies of the transgene. Lines 222 and 648 were used further and entered into the MGI database. (J:106642)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
