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The construct encodes a bidirectional reporter transgene. The cDNA of the chemokine KC (Cxcl1, from +2 to +140) was cloned into the pBI-G vector (Clontech). The vector contains a tetracycline responsive element consisting of seven copies of the tet-operator (tetO) that is between 2 CMV minimal promoters which are silent in absence of an activator (tTA or rtTA) sequence. When activated, one promoter drives expression of Cxcl1 while the other promoter drives expression of a beta-galactosidase reporter gene. (J:73096)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
